Fig. 2

Lack of ClC-2 reduced AngII-induced HBVSMC proliferation. a and b Cells were transfected with ClC-2 siRNA (siClC-2; 20 nM) or negative siRNA (negative; 20 nM) for 48 h in prior to angiotensin II (AngII) treatment (10^− 7 M) for another 48 h. Cell proliferation was determined using the CCK-8 assay (a) and BrdU incorporation (b). c and d The protein expressions of PCNA (c) and Ki67 (d) were detected using western blotting. **p < 0.01 vs. control, ^##p < 0.01 vs. AngII alone, n = 5