Fig. 4

ClC-2 inhibition attenuated the AngII-induced activation of Wnt/β-catenin signaling. a through f HBVSMCs were transfected with ClC-2 siRNA (siClC-2; 20 nM) or negative siRNA (negative; 20 nM) and then stimulated with angiotensin II (AngII; 10^− 7 M) for 48 h. Shown are the western blotting results for β-catenin phosphorylation (a), β-catenin cytosol (b) and nuclear protein (c) levels, GSK-3β phosphorylation (d), and survivin (e) and cyclin D1 (f) protein expression. g Quantitative real-time PCR analysis of Wnt3a and Wnt5a mRNA expression. h The cells were treated with recombinant Wnt3a (100 ng/ml) for 48 h. Wnt3a expression was examined using quantitative real-time. i Viability of HBVSMCs transfected with ClC-2 siRNA followed by co-incubation with recombinant Wnt3a and AngII. **p < 0.01 vs. control, ^##p < 0.01 vs. AngII alone, ^$$p < 0.01 vs. AngII+siClC-2, n = 4