Fig. 1

Effects of droxinostat on cell viability in colon cancer cells. a – HT-29 cells were treated with the indicated concentrations of droxinostat. The viability of the cells was determined using the MTT assay (left panel). The IC₅₀ of droxinostat was calculated using GraphPad software (right panel). Each point represents the mean ± SD of three independent experiments. b – Droxinostat with the indicated concentrations was assessed using a clonogenic assay with 500 cells in each well. The significance was determined using one-way ANOVA. c – HT-29 cells were incubated with droxinostat for 48 h. The indicated protein was detected via western blot. d – Knockdown HDAC3 by siRNA in HT-29 cells. e – A clonogenic assay using HDAC3 knockdown cells with 250 cells in each well. **p < 0.01 vs vehicle or control