Fig. 3

Droxinostat induced oxidative stress in colon cancer cells. a and b – HT-29 cells were treated with droxinostat for 12 and 24 h. ROS production was measured using the probe DCFDA and analyzed via flow cytometry. The levels of ROS production are expressed as mean fluorescence intensity (MFI) and the means ± SD of three independent experiments. H₂O₂ treatment was used as a positive control. c – HT-29 cells were incubated with droxinostat for 24 h. The expressions of SOD1, SOD2 and catalase were measured via western blot. GAPDH was used a loading control. d – HT-29 cells were incubated with MitoSOX Red 24 h after droxinostat treatment. Representative flow charts are shown, and the MFI is presented. e – phospho-Histone H2AX (pH2AX) staining was done 6 h after cells were incubated with droxinostat. DAPI was used to stain the nuclei. Representative pictures are shown (original magnification, 600×). **p < 0.01 vs Veh