Fig. 3

Notch signaling induces IκBα degradation through Asb2. a CCRF-CEM cells and MOLT-4 cells transduced with lentivirus carrying vehicle vector, Asb2, control shRNA or Asb2-shRNA2 were analyzed for their level of total IκBα. Untransduced cells served as the control. The levels of IκBα protein were then analyzed. **P < 0.01 versus empty vector control. CCRF-CEM cells and MOLT-4 cells were treated with DMSO (vehicle) or 10 μM GSI for 24 h. The total protein b and mRNA c levels of IκBα were then analyzed. Untreated cells served as the control. **P < 0.01 and *P < 0.05 versus DMSO control. d CCRF-CEM cells and MOLT-4 cells were treated with DMSO (vehicle) or 10 μM MG132 for 24 h. Untreated cells served as the control. The total protein levels of IκBα were then analyzed. **P < 0.01 and ***P < 0.001 versus DMSO control. The data are presented as the means ± SD. Each assay was conducted in triplicate