Fig. 2
From: Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element
Promoter analysis of mouse Dnmt1 in NIH3T3 and CCE cells using the luciferase assay. a Schematic structures of mouse Dnmt1 gene constructs used in this study. b, c, e and f – Oct4 enhanced the promoter activity of mouse Dnmt1. Oct4-pcDNA3.1 (100 ng) plasmid was co-transfected with a series of sequential deletion constructs (Dnmt1 P1–4) and a mutant of the mouse Dnmt1 promoter (500 ng/well) into NIH3T3 and CCE cells. d and g 10, 50, 100, 200, and 500 ng of Oct4-pcDNA3.1 expression plasmid was co-transfected with mouse Dnmt1-P3 promoter (500 ng/well) into NIH3T3 cells (d) and CCE cells (g). The total amount of the transfected plasmid, including the pRL-TK control vector (100 ng/well), was adjusted to 1.0 μg with pcDNA3.1 empty vectors. Firefly and Renilla luciferase activities were measured 48 h after the transfection. The relative luciferase activity was calculated by dividing the activity of firefly luciferase by the activity of Renilla luciferase. The data are presented as the means ± SD for triplicate transfections