Fig. 3
From: Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element
The chromatin immunoprecipitation assay was conducted to confirm the binding of Oct4 to the promoter of mouse Dnmt1 in vivo. a Enrichment results for Dnmt1 determined via ChIP-Seq. The different colors indicate the experiment type, and each square represents a list. Enriched terms are highlighted in circles and three terms are annotated. The brightness indicates the level of local similarity between the lists. b Anti-RNA Pol II and anti-Oct4 antibodies precipitated proteins bound in vivo to the specific amplified sequence of the mouse Dnmt1 promoter (the region between − 554 and − 294 bp). Non-specific IgG (negative control antibody) failed to do. A 213-bp length of PCR products were resolved on 1.5% agarose gel and stained with ethidium bromide to visualize the bands. c Analysis of the Dnmt1 promoter physically associated with Dnmt1 using ChIP-qPCR assay in the CCE cells