Fig. 4
From: Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs11658-018-0104-2/MediaObjects/11658_2018_104_Fig4_HTML.png)
The effect of mouse Oct4 knockdown in CCE cells. a and b CCE cells were transfected without (a) or with (b) the negative control alone for 48 h. c More than 85% of the cells were successfully transfected with Alexa Fluor Red Fluorescent Oligo. d, e and f The self-renewal and undifferentiated state of CCE cells were not maintained when transfected with the three siRNA of Oct4 – R1 (d), R2 (e) and R3 (f) when tested 48 h after the transfection. The arrows indicate the normal CCE mouse ES cells. The images were photographed with Leica DMI3000B at 200× magnification. Scale bar = 20 μm