Fig. 3
Identification of mesenchymal tissue stem cells (MSCs) in the cartilage proliferation model. CD44 and CD90 are markers for MSCs of auricular cartilage and Ki-67 protein is a cellular marker for proliferation. a, d, g: Biochemical evaluation of rabbit perichondrium tissue immunostained with anti-CD90 peroxidase-conjugated antibody; b, e, h: biochemical evaluation of rabbit perichondrium tissue immunostained with anti-CD44 peroxidase-conjugated antibody; c, f, i: biochemical evaluation of rabbit perichondrium tissue immunostained with anti-Ki67 peroxidase-conjugated antibody. a-c: Pre-injection of bFGF; d-f: week 1 post bFGF-injection; g-i: week 2 post bFGF-injection. A small number of CD44-positive and CD90-positive cells was observed in the noninjected group (a, b), but a large number of CD44-positive and CD90-positive cells was observed 1 week after the injection of bFGF. Most of spindle- and spider-shaped cells in the perichondrium layer stained positive for CD44 and CD90 (d, e). These positive cells decreased in number as the perichondrium layer thinned 2 weeks after the injection (g, h). Ki-67-positive cells were scarcely observed in noninjected controls or one day after injection (c). During week 1, Ki67-positive perichondrial cells were observed in the outer layer from the perichondrial inner-layer/outer-layer boundary (f). Only perichondrial cells stained positive for the Ki-67 marker in the superficial layer of the perichondrium during week 2 (i). “Reprinted with permission from (Yagishita, M. 2013. Involvement of mesenchymal tissue stem cells and aquaporin 1 in rabbit auricular cartilage regeneration from perichondrium with full reference. Journal of Kanazawa Medical University, 38, 43–52.). Copyright (2013) publication administration of Kanazawa Medical University”