Fig. 4

IL-33 increased expression of MMP2 and MMP9 via STAT3 signaling in NR8383 cells. (a and b) Levels of MMP2 (a) and MMP9 (b) in the medium at 24 h after IL-33 treatment with indicated concentrations in NR8383 cells. (c-e) Protein (c) and mRNA (d and e) levels of MMP2 and MMP9 at 24 h after IL-33 treatment with indicated concentrations in NR8383 cells. (f) Protein levels of p-STAT3 and STAT3 at 24 h after IL-33 treatment (400 pg/mL) in primary AMs. (g and h) Levels of MMP2 (g) and MMP9 (h) in the medium at 24 h after IL-33 (400 pg/mL) or stattic treatments in NR8383 cells. (i-k) Protein (k) and mRNA (i and j) levels of MMP2 and MMP9 at 24 h after IL-33 (400 pg/mL) or stattic treatments in NR8383 cells. (l) Protein levels of p-MAPK, MAPK, and p65 (within nucleus) at 24 h after IL-33 (400 pg/mL) or stattic treatments in NR8383 cells. (m) Levels of TNF-α, IL-6, IL-10, and IFN-γ in the medium at 24 h after IL-33 (400 pg/mL) or stattic treatments in NR8383 cells. Data are shown as means±SD (three independent repeats). The relative mRNA or protein level was normalized to 0 pg/ml (c-e) or PBS group (i-k). One-way ANOVA was used in the statistical analyses. * p < 0.05, ** p < 0.01, *** p < 0.001