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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1

Fig. 2

Effects of DKK1 silencing on cell proliferation, invasion and apoptosis in RASFs. RASFs were transfected with si-DKK1 or si-NC for 48 h. (a) Protein and mRNA expression of DKK1 was determined by qRT-PCR and Western blot, respectively. (b) Cell proliferation was assessed by BrdU-ELISA assay. (c) Invasion was assessed by Transwell assay after 6 h. (d) Cell apoptosis was measured by flow cytometric analysis of cells labeled with Annexin-V/PI double staining. All data are presented as mean ± SEM, n = 6. ##P < 0.01 vs. si-NC

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