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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1

Fig. 6

Overexpression of DKK1 partially blocked the effects of miR-613 mimic on cell proliferation, invasion and apoptosis in RASFs. RASFs were transfected with miR-613 mimic either with or without pcDNA3.1-DKK1 vector. (a) Protein expression of DKK1 was determined by Western blot. α-tubulin was detected as a loading control. (b) mRNA level of DKK1 was determined by qRT-PCR. (c) Cell proliferation was assessed by BrdU-ELISA assay. (d) Invasion of RASF cells was assessed by Transwell assay after 6 h. (e) Cell apoptosis was measured by flow cytometric analysis of cells labeled with Annexin-V/PI double staining. All data are presented as mean ± SEM, n = 6. ##P < 0.01 vs. miR-613 mimic + pcDNA3.1

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