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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: miR-29b-3p regulated osteoblast differentiation via regulating IGF-1 secretion of mechanically stimulated osteocytes

Fig. 4

miR-29b-3p regulated IGF-1 secretion of MLO-Y4 osteocytes, and after the osteocytes were transfected with miR-29b-3p mimic or miR-29b-3p inhibitor, the osteocytes’ conditioned culture medium influenced osteoblastic differentiation of MC3T3-E1 cells. a miR-29b-3p mimic decreased IGF-1 secretion of osteocytes, and the miR inhibitor increased the IGF-1 secretion (n = 6). The conditioned medium of osteocytes treated with miR-29b-3p mimic reduced ALP activity of osteoblasts (b), BMP-2 protein (c) and Runx 2 mRNA (d) levels of osteoblasts (n = 6). In contrast, the conditioned medium of osteocytes treated with miR-29b-3p inhibitor enhanced ALP activity of osteoblasts (b), BMP-2 protein (c) and Runx 2 mRNA (d) levels of osteoblasts (n = 6). *P < 0.05 and **P < 0.01, compared with the unstrained control group, or between the indicated groups

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