Fig. 5
From: MiR-214 sensitizes human colon cancer cells to 5-FU by targeting Hsp27
Hsp27 blocks the effect of miR-214. a, b and c – HT-29 and LoVo cells were transfected with ASO control, miR-214 ASO, miR-214 ASO + siRNA control or miR-214 ASO + Hsp27 siRNA. Protein expression of Hsp27 was detected via western blot (a and b) and IC50 values were detected using CCK-8 assays (c). d, e and f –HT-29 and LoVo cells were transfected with mimics control, miR-214 mimics, miR-214 mimics+pcDNA3 control or miR-214 mimics+pcDNA3-Hsp27.Protein expression of Hsp27 was detected via western blot (d and e) and IC50 values were detected using CCK-8 assays (f). Data represent the means ± SEM from three independentexperiments. *compared to control, p < 0.05;#compared tomiR-214 ASO + siRNA control, p < 0.05; &compared to miR-214 mimics+pcDNA3 control, p < 0.05