Fig. 3

Expression levels of related genes during differentiation induction in the 2D environment. (a) qRT-PCR analysis of Sox9, ACAN and collagen II on days 7, 14 and 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2 and annexin V on days 7, 14 and 21 during induction. The results were normalized to B2M mRNA expression. Values are means ± SD (n = 3). (c) Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during induction. (d) Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 21 during induction. Significant differences from the control group (non-transfection cells) are indicated by *p < 0.05 or **p < 0.01; differences between IHH and SHH transfection groups are indicated by ^#p < 0.05 or ^##p < 0.01