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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells

Fig. 3

Effect of MTNR1A and MTNR1B knockdown on testosterone secretion in mLTC-1. a. mLTC-1 cells were treated with 0, 0.5, 1.0 and 5.0 IU/ml of hCG for 6 h before detection of testosterone level using ELISA. The results indicate that 1 IU/ml is the optimal dose for testosterone synthesis. b and c. ELISA results of testosterone secretion after interference of MTNR1A or MTNR1B using siRNA in mLTC-1. d. ELISA result of testosterone secretion after interference with both MTNR1A and MTNR1B in mLTC-1. Data are presented as mean ± SEM of triplicate experiments. * P < 0.05, ** P < 0.01 (Student’s t-test)

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