Fig. 4
From: Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells
MTNR1A and MTNR1B regulated steroidogenic gene expression in mLTC-1 cells. a. mLTC-1 cells after transfection of si-Mtnr1a and si-Mtnr1b were treated with 1 IU/ml hCG for 6 h before monitoring of mRNA expression levels of p450scc, P450c17 and StAR using qPCR assay. b. Western blotting results of p450c17 and StAR protein expression in mLTC-1 after knockdown of MTNR1A and MTNR1B using specific siRNA