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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells

Fig. 5

Effects of MTNR1A and MTNR1B knockdown on endoplasmic reticulum stress. a-c. Relative mRNA expression of endoplasmic reticulum (ER) stress genes, Grp78, Chop, ATF4, Xbp1 and IRE1, was evaluated using qPCR assay after treatment with the siRNAs against MTNR1A and MTNR1B for 48 h in mLTC-1. The mRNA levels were normalized to that of Gapdh. Data are presented as the mean ± SEM of three triplicates. *P < 0.05, **P < 0.01 (Student’s t-test). d-f. Western blotting analyses of Grp78, ATF4 and phospho-IRE1 expression in mLTC-1 after knockdown of MTNR1A and MTNR1B using specific siRNAs

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