Fig. 3

CircDENND2A functions as an efficient miR-625-5p sponge in glioma cells. a Schematic drawing showing the putative binding sites for miRNAs in circDENND2A. b Fold changes of the 12 miRNAs in U87MG cells with overexpression of circDENND2A versus empty vector (EV) were determined by qPCR. c The levels of circDENND2A captured by biotinylated miR-625-5p or miR-Ctrl from U87MG cell lysates were measured by qPCR. d The luciferase activities of wild-type or mutant pGL3-control-circDENND2A in U87MG cells transfected with miR-625-5p or miR-Ctrl. e The levels of miR-625-5p in hypoxic or normoxic U87MG, A172, and SVGp12 cells with or without knockdown of circDENND2A were measured by qPCR. f The mRNA levels of AKT2 in U87MG cells transfected with circDENND2A or EV and miR-625-5p or miR-Ctrl were determined by qPCR. Data are shown as mean ± SD (three independent repeats). * p < 0.05, ** p < 0.01, *** p < 0.001