Fig. 2
From: Upregulation of miR-29b-3p protects cardiomyocytes from hypoxia-induced apoptosis by targeting TRAF5
Hypoxia-induced AC16 cell injury is attenuated by miR-29b-3p overexpression. AC16 cells were transfected with miR-29b-3p or miR-NC before culture under hypoxic conditions. Non-transfected AC16 cells were cultured under normoxic conditions as a blank group. a – The expression of miR-29b-3p was detected using quantitative real time PCR. b – CCK-8 assay was performed to measure cell viability. c and d – Flow cytometry with Annexin V/PI staining and Hoechst 33258 staining were used to assess cell apoptosis. Each experiment was repeated three times. ***p < 0.001 vs. miR-NC or blank