Fig. 4

Effect of curcumin on PD-induced p-TAK1, p-JNK and p-p38 activation in HMrSV5 cells. Cells were divided into control group (cells treated with DMEM/F12 medium containing 0.5%FBS), three PD groups (cells treated with 1.5% Dianeal, 2.5% Dianeal and 4.25% Dianeal) and three 4.25% Dianeal + curcumin group (cells treated with 4.25% Dianeal plus curcumin of 20, 40 and 80 μM). After incubation for 24 h, cells were lysed using RIPA buffer for western blot assay, respectively. Representative immunoblots of p-TAK1, p-JNK, p-p38 and β-actin in HMrSV5 cells under various treatments is shown in (a, b). β-actin served as a loading control. After normalizing to protein bands grayscale of β-actin, relative intensity of p-TAK1 (c, d), p-JNK (e, f) and p-p38 (g, h) are shown in HMrSV5 cells under various treatments. *P < 0.05 vs. control group, #P < 0.05 vs. 4.25% Dianeal group