Fig. 4

TCTN1 knockdown inhibited cell proliferation and induced apoptosis in ESCC. EC9706 and TE-9 cells were transfected with siTCTN1 or siNC, respectively. a qRT-PCR and b western blot analysis were performed to measure the TCTN1 expression at mRNA and protein levels, respectively. c CCK-8 assay was used to analyze cell proliferation in EC9706 and TE-9 cells. d Flow cytometry with double Annexin V/PI staining was used to detect the ESCC cell apoptosis. ***p < 0.001 vs. siNC