Table 2 Densitometric analysis of TGF-β1 protein expression levels
From: The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro
Untreated | Negative control | gRNA1 | gRNA2 | gRNA3 | |
|---|---|---|---|---|---|
TGF-β1 | 122.45 ± 0.27 | 177.34 ± 0.56 | 126.01 ± 0.39 | 2.37 ± 0.16 | 5.19 ± 0.13 |
GAPDH | 635.32 ± 0.83 | 679.98 ± 0.26 | 658.72 ± 0.76 | 654.75 ± 0.28 | 626.64 ± 0.68 |
Homogenization | 99.26 ± 0.34 | 134.31 ± 0.40 | 98.52 ± 0.30 | 1.87 ± 0.13 | 4.26 ± 0.12 |