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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: GRP78 regulates milk biosynthesis and the proliferation of bovinemammaryepithelial cells through the mTOR signaling pathway

Fig. 4

GRP78 is a positive regulator of the mTOR signaling pathway. a BMECs were transfected with pcDNA3.1-GRP78 vector or GRP78siRNA. Cells transfected with the empty vector (pcDNA3.1) or scramble siRNA were used as negative controls. The indicated protein levels were detected using western blotting analysis. β-actin served as a loading control. b Relative fold changes inGRP78 protein levels (protein/β-actin) in (a) were quantified using grayscale scanning. c and d Relative fold changes of indicated protein levels (protein/β-actin) were quantified using gray scale scanning after over expression (c) or silencing (d) of GRP78

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