Fig. 2

MiR-7-5p inhibits the proliferation, migration and invasion of A549 and SPCA-1 cells. a – qPCR assay confirming the transfection efficiency of the miR-7-5p mimic. b – MiR-7-5p suppressed the proliferation of A549 and SPCA-1 cells. Adherent A549 and SPCA-1 cells were cultured with or without miR-7-5p for 24, 48, 72 and 96 h, and the cell viability was then deteed using the MTT assay. c and d – MiR-7-5p inhibited the migration of A549 and SPCA-1 cells. Confluent cells were starved with serum-free medium and scratched with a pipette tip. After washing with PBS, the cells were cultured with or without miR-7-5p for 24 h. Representative images of the same field were photographed (100× magnification) at 0 h and 24 h. Representative images and quantitative data are shown in C and D, respectively. e and f – MiR-7-5p suppressed the invasion of A549 and SPCA-1 cells. A total of 2 × 10⁴ cells was seeded in the upper chamber of the transwell and treated with or without miR-7-5p for 24 h. After removing the cells on the inner wall of the upper chamber, the invading cells were photographed (100× magnification). Representative images and quantitative data are shown in E and F, respectively. The data were analyzed with GraphPad Prism 5.0. The data are presented as the means ± SEM, n = 3. ***p < 0.001 compared with the NC mimic group. g – MiR-7-5p downregulated EMT markers in A549 and SPCA-1 cells. β-actin was used as a loading control. The quantitative data were western blotting assays were measured with ImageJ software. Data are the ratios of EMT marker to β-actin