Fig. 3

MiR-376c-3p targets the 3′-UTR of ING5. (a) Diagram of the predicted miR-376c-3p binding site in the 3′-UTR of ING5. (b) The luciferase reporter assay was performed to examine whether miR-376c-3p directly binds to the ING5 3′-UTR in HEK293T cells. (c) The protein expression of ING5 was analyzed in PC-12 and SH-SSY5Y cells subjected to OGD. d and e—The mRNA (d) and (e) protein expression levels of ING5 were measured in PC-12 and SH-SY5Y cells transfected with mimic or miR-NC and subjected to OGD. f and g—The mRNA (f) and (g) protein expression levels of ING5 were determined in PC-12 cells transfected with anti-miR-376c-3p or anti-miR-NC and subjected to OGD. Data are expressed as means ± SD. **p < 0.01 vs. miR-NC; ^##p < 0.01 vs. anti-miR