Fig. 3
From: The long noncoding RNA LINC00483 promotes lung adenocarcinoma progression by sponging miR-204-3p
LINC00483 acts as a sponge of miR-204-3p. a The expression of LINC00483 in the cytoplasm and nucleus of A549 and PC-9 cells was measured using real-time PCR. b Fluorescence in situ hybridization assay was performed to determine the subcellular localization of LINC00483. c and d The correlation between LINC00483 (c) and miR-204-3p (d) was validated using the dual-luciferase assay. e LINC00483 and miR-204-3p were enriched via RNA immunoprecipitation with Ago2 antibody. SNRNP70 was used as a control. f The RNA levels of LINC00483 and miR-204-3p after LINC00483 overexpression were measured using real-time PCR. g and h The expression of miR-204-3p in tumor and normal tissues was measured using real-time PCR, and a negative correlation between LINC00483 level and miR-204-3p expression was observed, **p < 0.01; ***p < 0.001