Fig. 3

Existence of an ERE within the SREBP2 promoter. a ChIP analysis was performed using anti-ERα or anti-RNA polymerase II antibody to ascertain the existence of the ERE in the promoter of the SREBP2 gene. The PCR results show that one fragment containing the putative ERE could be precipitated after treatment of HepG2 and HuH-7 with E₂ (10^− 7 mol/l) for 24 h. b The pulled-down band was excised from the gel and sequenced. SREBP2: sterol regulatory element-binding protein; E₂: estradiol; ERE: estrogen response element; ChIP: chromatin immunoprecipitation