Fig. 5

SOX30 inhibited the activation of Wnt/β-catenin signaling in prostate cancer cells. a The effect of SOX30 overexpression on active β-catenin expression was determined using western blotting. PPC-1 and PC-3 cells were transfected with SOX30 expression vector and incubated for 48 h. b The effect of SOX30 overexpression on Wnt/β-catenin signaling was assessed with a TCF/LEF luciferase reporter assay. PPC-1 and PC-3 cells were co-transfected with SOX30 expression vector and TCF/LEF luciferase reporter vector and incubated for 48 h. c The effect of miR-653-5p inhibition on active β-catenin expression was measured using western blotting. PPC-1 and PC-3 cells were transfected with miR-653-5p inhibitor or NC inhibitor and incubated for 48 h. d The effect of miR-653-5p inhibition on Wnt/β-catenin signaling was assessed with a TCF/LEF luciferase reporter assay. PPC-1 and PC-3 cells were co-transfected with TCF/LEF luciferase reporter vector and miR-653-5p inhibitor or NC inhibitor and incubated for 48 h (n = 5, *p < 0.05)