Fig. 1

LPS stimulation promotes GNAS expression in HCC cells, and GNAS knockdown inhibits LPS-induced IL-6 expression. a GNAS protein expression levels in different tissues were detected by Western blotting. b GNAS protein expression levels in the indicated HCC cell lines and HL-7702 human normal liver cells were detected by Western blotting. c and d HepG2 cells were treated with the indicated LPS (c) or DEN (d) for 12 h, and then GNAS protein expression levels were detected by Western blotting. e and f HepG2 cells were transfected with si-NC or si-GNAS for 24 h and then treated with LPS (5 μg/ml) for 12 h. The relative expression levels of the indicated mRNAs were analyzed by qRT-PCR (e). The expression levels of IL-6 in the culture supernatants were measured by ELISA (f). Data are represented as means ± SD (n = 3; ^*represents P < 0.05)