Fig. 1

Oxidative stress up-regulated miR-711 expression. a H₂O₂ increased the expression of miR-711. H9c2 cells were cultured and treated with H₂O₂ at the indicated concentrations for 24 h (left panel), or with 300 μmol/L H₂O₂ at indicated hours (right panel). RNA was extracted using a microRNA extraction kit and miR-711 expression was detected by qRT-PCR. b AA induced cell apoptosis/death and up-regulated miR-711. H9c2 cells were treated with AA at the indicated concentrations for 3 h followed by culture in DMEM-CM for another 3 h. miR-711 expression was determined by RT-PCR (left panel). Cell apoptosis/death for cells treated with 20 μM AA was measured by Annexin-V and PI staining and flow cytometry (right panel). c CoCl₂ treatment upregulated miR-711 expression. H9c2 cells were treated with CoCl₂ at the indicated concentrations for 24 h (left panel), or treated with 500 μM CoCl₂ for the indicated durations (right panel). miR-711 expression was detected by qRT-PCR. d Hypothermal H/R increased miR-711 expression. H9c2 cells were cultured overnight and subjected to cold hypoxia environment (0.5% O₂ at 10 °C) for 18 h followed by 24 h reoxygenation at normal cell culture conditions. miR-711 was detected by qRT-PCR. All qRT-PCR data were presented as 2⁻∆∆Ct. Snord61 was used as a loading control. n = 3, *P < 0.05, ** P < 0.01, *** P < 0.05