Fig. 6

NFКB regulated miR-711 expression a P-p65 expression. Total protein was extracted from cells treated with AA and p-p65 expression was determined by Western blotting. Left: Representative images of Western blotting: Right: semi-quantitative results of Western blotting. b Inhibition of the NFКB and PI3K pathways decreased miR-711 expression. Cells were treated with NFКB inhibitor Bay 11–7082 or PI3K inhibitor LY294002 for 1 h prior to cold H/R. miR-711 was detected by qRT-PCR. c Treatment with Bay 11–7082 and Ly 294002 reduced cell death. Cells were pre-treated with Bay 11–7082 and LY 294002 for 1 h and then treated with H₂O₂ and CoCl₂. Cell death was detected in an IncuCyte system as described in (5D). d Pretreatment with Bay 11–7082 and Ly 294002 reduced miR-711 induced by H₂O₂ and CoCl₂. Cells were treated as described in (6C). miR-711 was measured by qRT-PCR. e The effect of HIF-1α siRNA on pre-miR-711 expression. Cells were treated as described in (b). Pre-miR-711 was measured by qRT-PCR. f The effect of Bay 11–7082 and Ly 294002 on Pre-miR-711. Pre-miR-711 expression was measured by qRT-PCR. n = 3, * P < 0.05