Fig. 3

MiR-483-5p influenced cardiomyocyte apoptosis and oxidative stress following hypoxic injury. AC16 cells were transfected with miR-483-5p mimic or inhibitor, followed by 24 h hypoxia treatment. a Quantitative real-time PCR was used to determine the expression of miR-483-5p. b Cell viability was measured using a CCK-8 assay. c Cell apoptosis was analyzed using flow cytometry with double Annexin V FITC-PI staining. d through f The content of MDA and the activities SOD and CAT were measured using commercial assay kits. Data are indicated as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, as compared with miR-NC