Fig. 3

MiR-206 directly targets the 3′-UTR of CORO1C. a The sequence of human miR-206 and the predicted binding sites with miR-206 within the CORO1C 3′-UTR are shown. b MiR-206 suppresses CORO1C expression in A549 cells. The A549 cells were treated with NC mimic or miR-206 mimic for 24 h, and CORO1C expression was evaluated by western blotting assay. β-actin was used as a loading control. c MiR-206 represses CORO1C mRNA in A549 cells. The A549 cells were cotransfected with luciferase plasmids containing wild-type (WT) CORO1C 3′UTR or mutant-type (Mut) CORO1C 3′UTR. The cells were also treated with miR-206 mimic at the same time. The cells were lysed to measure the relative luciferase activity. Quantitative data are presented as mean ± SEM. ***P < 0.001 compared with the NC mimic group. d CORO1C expression in NSCLC tissues and corresponding normal mucosa tissues were detected by q-RT-PCR. e CORO1C expression in a panel of human lung cell lines and human lung epithelia BEA-2B cells was evaluated by q-RT-PCR. CORO1C expression in BEA-2B was set as 100%. Quantitative data are presented as the mean ± SEM. ***P < 0.001 compared with BEA-2B cells