Fig. 4

CORO1C overexpression attenuates miR-206-mediated inhibitory effect on A549 cells. a CORO1C vector transfection increased CORO1C expression. The cells were treated with CORO1C vector or NC vector for 24 h, then the cells were collected for q-RT-PCR assay. b CORO1C vector transfection decreases miR-206-mediated inhibition effect on A549 cell proliferation. The cells were transfected with NC vector and CORO1C vector, and then treated with miR-206 mimic for 24 h. Then the cells were subjected to MTT assay. c and d) CORO1C overexpression rescues miR-206-induced effect on the horizontal migration of A549 cells. A549 cells were transfected with NC vector or CORO1C vector, and then applied for wound healing assay stimulated with miR-206 mimic. Representative images (100× magnification) and the quantitative data are shown in c and d, respectively. (e and f) CORO1C vector attenuates miR-206-mediated inhibitory effect on the vertical migration and invasion of A549 cells. A549 cells were transfected with NC vector or CORO1C vector, and then subjected to transwell migration and transwell invasion assays. Representative images (100 × magnification) and the quantitative data are shown in e and f, representatively. g CORO1C vector rescues miR-206-induced inhibitory effect of EMT markers. The cells were transfected with CORO1C vector or NC vector. After 24 h the cells were collected and subjected to western blotting assay. Quantitative data are presented as mean ± SEM. *P < 0.05 and ***P < 0.001 compared with the NC vector group, ^#P < 0.05 and ^##P < 0.01 compared with the NC vector+ NC mimic group