Fig. 2
Evolution of spontaneous calcium activity parameters during W8B2+ CSC differentiation. a Types of spontaneous calcium activity recorded at the beginning of the differentiation process [day 2 after differentiation (1)] and at the end of the differentiation process [day 28 after differentiation (2)]. The graphs illustrate variations in [Ca2+]i represented by ΔF/F0 ratio, where F0 represents the basal fluorescence emitted by the GCaMP, and ΔF the difference between basal and actual fluorescence (F) at a given time point. b Bar graphs (1), (2), (3), (4) represent the evolution of the frequency, the peak amplitude, the duration and TTP (time to peak) of calcium oscillations, respectively, recorded during the 4 weeks of W8B2+ CSC differentiation. (N = 6 patients, n: represents the number of cells analyzed, AU: arbitrary unit, ns: not significant). *p < 0.05, **p < 0.01, ***p < 0.001 vs. week 1; one-way ANOVA followed by a Newman-Keuls test