Fig. 5
Role of SERCA and IP3 receptors on spontaneous calcium activity recorded in W8B2+ CSCs after 28 days of in vitro differentiation. a Effect of 5 µM xestospongin C (IP3 receptor inhibitor) and c 1 µM thapsigargin (SERCA inhibitor) on spontaneous calcium activity recorded in differentiated CSCs. Graphs illustrate variations in [Ca2+]i represented by changes in the ΔF/F0 ratio. ΔF = F − F0 (F: fluorescence emitted by GCaMP at a given time; F0: basal fluorescence emitted by GCaMP). Bar graphs (1), (2), (3) represent the evolution of the peak amplitude, the duration and the frequency of calcium oscillations, respectively, recorded from differentiated W8B2+ CSCs after treatment with xestospongin C (5 µM) (b) and thapsigargin (1 µM) (d). (N = 3 patients, n: represents the number of events (oscillations) recorded, AU: arbitrary unit, ns: not significant). *p < 0.05, **p < 0.01 vs. control; t-test paired followed by Wilcoxon test