Fig. 8

αII-spectrin depletion is associated with reduced expression of proteins involved in capillary tube assembly and formation. HMEC-1 cells were transfected with plasmids expressing either non-relevant shRNA (Nr shRNA) or shRNA directed against αII-spectrin (Sp shRNA). Western blot analyses were performed 72 h after transfection on cell lysates (20 μg). Spectrin depletion does not disturb expression of VASP, ABI-1 or β1-integrin. The expressions of VE-Cadherin, MCAM and β3-integrin were significantly reduced in cells transfected with Sp shRNA