Fig. 2

Effect of different concentrations of jagged-1 and DAPT on mediated expression of IL-22. After the naïve CD4⁺ T cells were pretreated with a combination of factors, different concentrations of jagged-1 (0, 0.1, 0.5, 1, 5 µg/mL) were added to culture medium. a Flow cytometric analysis of the positive IL-22 cell proportion in different doses of jagged-1 treatment. b, c IL-22 proteins were determined by Western blotting. d Naïve CD4⁺ T cells were treated with the combination of factors, and exposure with or without jagged-1 (1 µg/mL), and different doses of DAPT added (0, 1, 5, 10, 20 µM). Then, the percentages of positive IL-22 cells were assayed by flow cytometry. e, f Following the treatment, expression of IL-22 protein was determined by Western blotting. Data are presented as the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001 vs. untreated control, ^##p < 0.01 vs. jagged-1 (1 µg/mL) without DAPT group