Fig. 7

Actin polymerization state and osteogenic differentiation of hASCs. a Cells were treated with osteogenic induction medium (containing different concentrations of JAS). They were then stained with alizarin red. b Western blot analysis for osteogenic markers of hASCs cultured in the osteogenic induction medium (containing different concentrations of JAS). Samples were collected after one week in culture. Quantification of western blot data was performed by Gel-pro software. The relative expression of these proteins was normalized to that of GAPDH. Results are expressed as mean ± SD; *p < 0.05. Scale bar = 100 µm