Fig. 4

Worenine promotes the degradation of HIF-1α and inhibites HIF-1α entry into the nucleus. HCT116 cells were treated with various concentrations of worenine (5, 10 and 20 μM) for 24 h. a The effect of worenine on nuclear HIF-1α expression in cells was investigated via western blotting analyses of nuclear protein extracts. b The effect of worenine on membrane HIF-1α expression in cells was investigated via western blotting analyses of membrane protein extracts. c After treatment with a protein synthesis inhibitor, the protein expression levels were determined using western blotting. d Immunofluorescence staining of HIF-1α with or without worenine. The nucleus of the HCT116 cells was stained by DAPI. The data are means ± SEM. ^#p < 0.05, ^##p < 0.01 vs. control group