Fig. 1

Workflow of neurogenic differentiation procedure. Undifferentiated T-MSCs are dissociated from the plate and cultured in ultra-low attachment conditions in neurosphere media. A Representative images of spheres after 24 h (Day 1), 48 h (Day2) and 72 h (Day 3). The resultant neurospheres are dissociated and re-plated on PDL/Laminin coated plates in basal neuronal media. B Representative images of cells after an additional 72 h (Day 6). On the sixth day, media is replaced with neuronal maturation medium and cells are further differentiated through Day 28. C Representative images of cells after a total of 11 days and D 28 days. These results were obtained from at least 5 donors in triplicates in multiple independent experiments.