Fig. 10

RNA-seq revealed that JMJD1A knockdown ameliorates injury by FOS/FOSB under high glucose and hypoxia. a–b Heatmaps of differentially expressed genes related to response to oxidative stress (a) and inflammatory response, regulation of immune response (b). c–d Venn diagram shows genes regulated by JMJD1A only under high glucose and hypoxia in HUVECs. e PPI network of the high-glucose- and hypoxia-induced HUVECs by silencing JMJD1A. The size of the nodes was positively related to the degree of nodes; red triangles indicate transcription factors, while blue nodes indicate DEGs. f–g Results of RNA-seq (f) and qRT-PCR (g) for core transcription factors. PPI, protein–protein interaction; HG, high glucose; HG + Hypoxia, combined stimulus with high glucose and hypoxia; NG, control; shCtrl, control shRNA