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Fig. 8 | Cellular & Molecular Biology Letters

Fig. 8

From: HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury

Fig. 8

Knockdown of JMJD1A in HUVECs decreases inflammation and oxidative stress injury induced by high glucose and hypoxia. a Knockdown of JMJD1A under high-glucose and hypoxic conditions in HUVECs. Western blotting analysis of JMJD1A levels (b, c) in HUVECs stably transduced with shJMJD1A and control scrambled shRNA (shCtrl), and cultured in normal or high-glucose and hypoxic conditions for 6, 12, 24, and 48 h. Cells were transfected with control shRNA or shJMJD1A for 48 h following combined stimulation, and qPCR (d) and ELISA for IL-6, IL-8, ICAM-1, and MCP-1 (e) were performed. Downregulation of JMJD1A decreased the secretion of IL-6, IL-8, and MCP-1, but did not affect ICAM-1 secretion. Assays for ROS (f and g), MDA (h), and SOD activity (i) are shown compared with controls. JMJD1A shRNA decreased the expression of ROS and MDA level, and increased SOD activity markedly compared with control shRNA-transfected cells. n=3; *p<0.05 and **p<0.01 vs. control shRNA-transfected cells. HG, high glucose; HG+Hypoxia, combined stimulus with high glucose and hypoxia; NG, control; shCtrl, control shRNA

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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