Fig. 1
From: MiR-26b-5p inhibits cell proliferation and EMT by targeting MYCBP in triple-negative breast cancer
MYCBP is a direct target of miR-26b-5p. A The binding site for miR-940 in the 3-′UTR of FOXO3 predicted by bioinformatics was presented. Luciferase reporter assay evaluation of the interaction between miR-26b-5p and the 3′-UTR of MYCBP mRNA. Luciferase activity was determined 48 h after co-transfection of luciferase constructs containing the wild-type (WT) or a mutated (MUT) 3′-UTR of MYCBP mRNA and miR-26b-5p mimics or miR-NC in MDA-MB-468 (B) and BT-549 (C) cells. Luciferase activity was determined 48 h after co-transfection of luciferase constructs containing the WT or a MUT 3′-UTR of MYCBP mRNA and miR-26b-5p inhibitor or inhibitor NC in MDA-MB-468 (D) and BT-549 (E) cells. Data represent the mean ± SD of three independent experiments. ***p < 0.001, compared with miR-NC or inhibitor NC