Fig. 3

FKBP25 was essential for ESD suppressing mTORC1 activity. Western blot analysis of MAP1LC3BII (a, b), mTORC1, phosphorylated mTORC1 (p-mTORC1, Ser2448) (c, d), P70S6K and phosphorylated P70S6K (p-P70S6K, Thr389) level (e, f) in A549 cells treated with FPD5 at 5 μM for 24 h after transfection with scramble siRNA or specific siRNA for FKBP25 (siFKBP25). g–k HEK293T cell line was transfected with plasmids FKBP25-WT and the deletion mutants of FKBP25 for 24 h followed by western blot analysis of the levels of p-mTORC1, p-P70S6K, MAP1LC3BII and GFP-FKBP25. l, m The interaction between FKBP25 and mTORC1 in wild-type HEK293T cells and the ESD-knockout cell line (ESD^−/−) treated with FPD5 (5 μΜ) for 24 h. Data are mean ± SEM. *p < 0.05, **p < 0.01, N.S., not significant, n = 3