Fig. 1
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs11658-021-00304-6/MediaObjects/11658_2021_304_Fig1_HTML.png)
HCG18 knockdown inhibited OS proliferation in vitro. A HCG18 expression in OS cell lines and in hFOB1.19. B Knockdown efficiency of HCG18 in OS cells. CCK8 (C and D), colony formation (E), EDU (F and G) assays performed with OS cells transfected with sh-HCG18 or sh-CON. H and I Cell apoptosis was determined in the OS cells by flow cytometry after transfection with sh-HCG18. Knockdown of HCG18 significantly induces apoptosis of OS cells. ** represents P < 0.01, *** represents P < 0.001. All experiments were repeated three times