Fig. 3
From: Construction and investigation of β3GNT2-associated regulatory network in esophageal carcinoma
β3GNT2 promotes ESCA cell growth, migration, and invasion in vitro. A Analysis of β3GNT2 mRNA expression by qPCR. B Analysis of β3GNT2 protein expression by Western blot. C Cell proliferation was determined by the CCK-8 assay. D Colony formation ability was measured by colony formation assay. E Cell apoptosis was assessed by flow cytometry. F Cell migratory potential was analyzed by Transwell migration assay. G Cell invasion capacity was detected by Matrigel invasion assay. Cells were transfected with the indicated plasmids. shNC, negative control shRNA; shRNAs, β3GNT2 shRNA lentiviral vector; Mock, empty plasmid; OV, pcDNA3.1/β3GNT2 plasmid. Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001