Fig. 7
From: Construction and investigation of β3GNT2-associated regulatory network in esophageal carcinoma
β3GNT2 is a downstream target of CREB1. A Transcription factors (TFs) of β3GNT2 predicted by KnockTF. B Correlation analysis between transcriptional activators and β3GNT2 based on the TCGA and GTEx databases. C Analysis of β3GNT2 expression through the TCGA and GTEx databases. D Correlation analysis of CREB1 mRNA expression and β3GNT2 mRNA expression in clinical ESCA samples. E Analysis of β3GNT2 mRNA expression by qPCR. F Analysis of β3GNT2 protein expression by Western blot. G CREB1 binding site in the β3GNT2 promoter region. H ChIP-qPCR analysis of CREB1 binding to the β3GNT2 promoter. I Dual-luciferase reporter activity assay. J–M The proliferation, colony formation, migration, and invasion of cells co-transfected with β3GNT2 overexpression plasmid and CREB1 shRNA were measured by CCK-8 assay (J), colony formation assay (K), Transwell migration assay (L), and Matrigel invasion assay (M). Cells were transfected with the indicated plasmids. WT, wild-type; MUT, mutant; OV, pcDNA3.1/β3GNT2 plasmid; sh-Ctrl, control shRNA; sh-CREB1, CREB1 shRNA lentiviral vector. Data are expressed as mean ± SD. NA, Data not available; *P < 0.05; **P < 0.01; ***P < 0.001