Fig. 8
From: Construction and investigation of β3GNT2-associated regulatory network in esophageal carcinoma
β3GNT2 expression is controlled by miR-133b. A Prediction of regulatory miRNAs of β3GNT2. B Heatmap showing the top 50 miRNAs negatively correlated with β3GNT2 in ESCA. C Analysis of the correlation between miR-133b and β3GNT2 expression based on the TCGA database. D Correlation analysis of miR-133b and β3GNT2 expression in clinical ESCA samples. E Analysis of β3GNT2 mRNA expression by qPCR. F Analysis of β3GNT2 protein expression by Western blot. G Putative miR-133b binding site in the β3GNT2 3′-UTR. H Dual-luciferase reporter activity assay. I–L The proliferation, colony formation, migration, and invasion of cells co-transfected with β3GNT2 overexpression plasmid and miR-133b mimics were measured by CCK-8 assay (I), colony formation assay (J), Transwell migration assay (K), and Matrigel invasion assay (L). M Diagram of the proposed mechanism showing how β3GNT2 regulates ESCA progression. OV, cells transfected with pcDNA3.1/β3GNT2 plasmid. Data are expressed as mean ± SD. #P > 0.05, *P < 0.05; **P < 0.01